The import requires an ASCII text file of the proper format. In the Genotyper software, use the "Make allele table" option (Apple-5), then select the Table tab to export it as an Excel table. Then open it in Excel and save it as tab-delimited Ascii. Move it to the PC where DNA·VIEW is.
Choose the Genotyper import (or Genomyx M.W. import) option of Import/Export, type in the full path of the subdirectory that contains the file(s) to import, then choose the first file to import from the menu of files.
The first column of the input file (Sample Info) is not used, which is an improvement compared to the old behavior which sometimes tried to interpret it as a lane number.
The report that is prepared includes the Sample Info presented side-by-size with the membrane roster. This is useful for checking.
You can now import several versions of the same data, using different reader numbers. (If you use the same reader number twice, the second import will replace the first one.) The second and subsequent import with different readers will be compared and any differences are mentioned in the report.
The spelling of locus names the column headings of the input file is not critical. If any input name is not identical with a locus name in DNA·VIEW, the import process suspends and asks you to select from the locus menu to clarify.
Quality control (i.e. K562) size checking is implemented, just as with Read.
Calculate crime allele frequencies simultaneously?
works in conjunction with
Count observed allele how many times?
Suppose some database has N=100 chromosomes, that Count how many? is 1, and that alleles 10 and 11 are the alleles that are observed at some locus in a crime stain analyzed with any of the stain calculation tools. Then frequencies are calculated as
allele | database frequency | "simultaneously?" frequency | |
no | yes | ||
10 | 0/100 | 1/101 | 1/102 |
11 | 5/100 | 6/101 | 6/102 |
Obviously the results are not very different whether you use this option or not. Although it is logical, since it is a bit complicated and makes little difference the recommended setting is no. (In almost every situation no is slightly conservative.)
DNA·VIEW will attempt (success not guaranteed) to resolve the
sizes consistently among all trios in a case if you choose the option
Make consensus size the same for multiple children etc.?
If you prefer to have the smaller allele for each person always listed first, go to Options, Pater report, sort alleles?, and choose y for Sort DNA alleles? and also for Sort with smaller allele first?
The customized order can be changed whenever a locus list is displayed. Highlight in red any locus, then use PageUp or PageDown to move the position of the locus within the list. Del moves the locus toward the end of the list.
The custom order can be accessed for DNA odds or DNA exclusion by using the # ("next locus") key.
www.dna-view.com
is the new name for
http://www.ccnet.com/~cbrenner.
There is also a new e-mail address
chb@dna-view.com
. (Web lore:
Actually anything of the form xyz@dna-view.com
works
just as well.)