|Import/Export|| Genotyper import
Hitachi StarCall import
Genomyx M.W. import
The interface with Genotyper and similar files has been refined in very useful ways with DNAVIEW version 23. Most importantly, you can import the roster. This means, if you already filled in the Genotyper spread-sheet Sample Info column, you won't have to enter the person identification ("accession") numbers again into the DNAVIEW membrane roster. The roster will be populated automatically, provided the input file conforms to any of several allowable possibilities:
|role-within-case (DNAVIEW style)||16080 C
|role-within-case (various acceptable styles)||16080 C2
Note: role-within-case requires that the case already be defined.
The way to label quality control samples is to use their DNAVIEW accession number code, such as 1-562.
A membrane is no longer required to have at least one ladder lane. This makes importing (above) a little simpler and more rational.
The lane report of Read the screen after digitization showing bands/lane etc. has been moved to the action menu in order to avoid the annoyance that it could be so big as to interfere with that menu.
Mutation history is a new command (DNAVIEW version 22.37) that analyzes the casework history to estimate mutation rates. This command is extremely useful for labs wanting to conform to AABB rules, or for research.
The output gives both a summary table of apparent mutation rates abbreviated form:
|Locus||est mut'ns per 1000||cases with 0 or 1 "exclsn"||poss mut'ns|
and a detail, showing every suspected mutation, e.g.:
|(108) Case 99123-10 Residual PI=2,000,000,000
from 13 consistent loci. Inconsistencies:
D3S1358 (b) M= 16 Ch= 18 16 Af= 16 17
(109) Case 99129 Residual PI=100,000,000 from 12 consistent loci. Inconsistencies:
D3S1358 (c) M= 15 17 Ch= 17 15 Af= 16
which gives the information that you need to refine the summary such as by excluding cases that are really primer dropout, or that are non-paternity, such as uncle cases.
Under Paternity Case, Options, choose the switch
Post DNA calculations to a Paradox file?
and be sure it is set to yes. (For PATER users, it will already be.) Once this is done, all cases analyzed by Paternity Case will in the future be available for analysis by Mutation history.
In order to make old cases available for analysis, re-run them. For a moderate number (<100) of old cases, it is reasonably easy using the batch select and batch run options of Paternity case. For a larger number, contact me for special help.
etc . . .
The user is successively asked to designate (by letter codes, e.g. de) pairs of columns to import as a database. The program makes a guess as to the locus based on the column headings if present, which the user can confirm or correct. The importing process is therefore very quick and painless.
Several databases corresponding to several loci imported from the same file will include internal notations so that the DNAVIEW program Create phenotype list (see below) will be able to reconstruct the multiple-locus genotypes and hence search for duplicates or for a suspect profile.
An old facility, but recently updated for efficiency and for STR's, DNAVIEW has the capability to maintain and search a convicted offender database.
A few pointless lines are now (ver 23.08) omitted from the Paternity case calculate report output, especially for STR's. The "Gjertson PI" is omitted when it is 0/0; documentation of delta and sigma are omitted when they are 0.
Membrane before version 23.11 apparently generated a harmless bug report every 100th attempt to create a membrane.